Molecular Characterization of Rhizoctonia Solani Ag-7 Causing Root Rot on Cotton Crop in India
نویسندگان
چکیده
Rhizoctonia solani (Kuhn) is one of the most important cotton crop pathogens. Rhizoctonia solani anastomosis groups (AGs) 7 and 4 are proven to be the most common pathogenic fungal strains on cotton crop. AG-7 can cause root rot in plants while damping-off of seedlings is most frequently attributed to AG-4. Sequencing of the ITS (Internal Transcribed Spacer) region of ribosomal DNA was performed on northern Indian isolates in order to determine their relatedness. Sequence analysis using BLAST revealed that rDNA ITS region of MV1 [JX576189.1] was at least 98% identical to that of other AG7 isolates (AY154305.1) on the GenBank database. Bio-molecular evaluation by means of electrophoretic profile of isozyme polyglacturonase and RFLPs of ribosomal DNA internal transcribed spacers were carried out. The isolates shared a common pectic zymogram while sequence analysis of rDNA showed a closer similarity to anastomosis group-7(AG-7). Based on morphological characteristics, hyphal fusion compatibility and sequence analysis, 98% of the isolates collected belonged to AG-7 and 2% of the isolates were AG-4 R.solani . This is the first report of R. solani AG-7 infecting cotton crop in Northern India.
منابع مشابه
Antagonistic Potential and Molecular Characterization of Trichoderma spp. against Rhizoctonia solani Infecting Ghost Pepper in Manipur, India
Bhut jolokia (Capsicum chinense Jacq.) is one of the world’s hottest chilli entered in the Guinness book of world record (2006). Many pest and diseases ruined Bhut jolokia and among them damping-off disease of seedling as well root and stem rot in young transplants caused by Rhizoctonia solani is one of the important limiting factor in cultivation and production (Ngullie and Daiho, 2013). Even ...
متن کاملStem Rot on Adzuki Bean (Vigna angularis) Caused by Rhizoctonia solani AG 4 HGI in China
During late August and early September 2011, stem rot symptoms were observed on adzuki bean plants (Vigna angularis) growing in fields located in Beijing and Hebei Province, China, respectively. In this study, four isolates were obtained from infected stems of adzuki bean plants. Based on their morphology, and sequence and polymerase chain reaction-restriction fragment length polymorphism (PCR-...
متن کاملWheat Genotype-Specific Induction of Soil Microbial Communities Suppressive to Disease Incited by Rhizoctonia solani Anastomosis Group (AG)-5 and AG-8.
ABSTRACT The induction of disease-suppressive soils in response to specific cropping sequences has been demonstrated for numerous plant-pathogen systems. The role of host genotype in elicitation of the essential transformations in soil microbial community structure that lead to disease suppression has not been fully recognized. Apple orchard soils were planted with three successive 28-day cycle...
متن کاملEffect of inoculum density and soil tillage on the development and severity of rhizoctonia root rot.
Rhizoctonia spp. cause substantial yield losses in direct-seeded cereal crops compared with conventional tillage. To investigate the mechanisms behind this increased disease, soils from tilled or direct-seeded fields were inoculated with Rhizoctonia spp. at population densities from 0.8 to 250 propagules per gram and planted with barley (Hordeum vulgare). The incidence and severity of disease d...
متن کاملSilver nanoparticles, a potential alternative to conventional anti-fungal agents to fungal pathogens affecting crop plants
Metallic silver nanoparticles have been reduced from silver nitrate by employing the extracellular enzymatic machinery of edible White button Mushroom (Agaricus bisporus). The physical properties of these particles, size and shapes have been determined using techniques like TEM, FTIR and XRD and were reported in our earlier report. But, what stands of paramount importance in the presen...
متن کامل